Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: Effect of reaction conditions

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Two active forms of Zymomonas mobilis levansucrase. An ordered microfibril structure of the enzyme promotes levan polymerization.

Fructansucrases, members of glycoside hydrolase family 68, catalyze both sucrose hydrolysis and the polymerization of fructose to beta-d-fructofuranose polymers. The resulting fructan polymers are distinguished by the nature of the glycosidic bond: inulin (beta-(2-1)-fructofuranose) and levan (beta-(2-6)-fructofuranose). In this study we demonstrate that Zymomonas mobilis levansucrase exists in...

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Sucrose utilization by Zymomonas mobilis: formation of a levan.

1. Molar growth-yield coefficients of Zymomonas mobilis for glucose, fructose, glucose plus fructose, and sucrose are reported. Yield coefficients for sucrose are appreciably lower than those for the equivalent concentrations of glucose plus fructose. 2. Only 2.6% of [U-(14)C]glucose supplied in the growth medium is incorporated into cell substance by Z. mobilis utilizing glucose as the energy ...

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Influence of fermentation conditions on levan production by Zymomonas mobilis CT2

Zymomonas mobilis produces two extracellular sucrases namely levansucrase (sac B) and sucrase (sac C). A mutant strain of Z. mobilis CT2 defective in sucrase sac C, constructed earlier by gene disruption, produced higher levels of levan (27.2 g L) than the parent strain B14023 (15.4 g L) from 200 g L of sucrose at 25°C and pH 5.0. Increasing fermentation temperature from 25 to 35°C enhanced eth...

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Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis.

Lactosucrose (4(G)-beta-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were 23 degrees C, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/...

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Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase.

His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge an...

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ژورنال

عنوان ژورنال: Journal of Molecular Catalysis B: Enzymatic

سال: 2015

ISSN: 1381-1177

DOI: 10.1016/j.molcatb.2015.05.011